Journal: Bioactive Materials

Article Title: HydroWrap for T2DM-Related Fractures: A smart H 2 S-delivery controller modulating Macrophage senescence

doi: 10.1016/j.bioactmat.2025.05.007

Figure Lengend Snippet: Macrophage Rejuvenation Enhances Angiogenesis and Osteogenesis. (a) Schematic illustration of the experimental approach to evaluate the impact of conditioned medium from macrophages on angiogenesis and osteogenesis. (b) Crystal violet staining of HUVECs in the wound healing assay, and (c) quantitative analysis of wound closure. (d) Representative images from the tube formation assay and (e) quantitative analysis of vessel area percentage. (f) Representative images of ALP and ARS staining in MC3T3-E1 cells, accompanied by (g) quantitative analysis of ALP activity and (h) mineralization levels. (i) Relative mRNA expression levels of angiogenesis-related genes in HUVECs and (j) osteogenesis-related genes in MC3T3-E1 cells (n = 6 per group). Statistical significance is indicated as follows: ∗, #, and $ denote p < 0.05 when comparing the Control, HydroWrap, and HydroWrap + NIR groups to the T2DM group, respectively.

Article Snippet: Additional kits, including the BCIP/NBT ALP Color Development Kit, ALP Assay Kit, ARS Staining Kit, and Enhanced Mitochondrial Membrane Potential Assay Kit with JC-1, were acquired from Beyotime Biotechnology (Jiangsu, China).

Techniques: Staining, Wound Healing Assay, Tube Formation Assay, Activity Assay, Expressing, Control

Journal: Calcified Tissue International

Article Title: Identification of a Novel Gene ARNT2 for Osteogenic Differentiation of Mesenchymal Stem Cells

doi: 10.1007/s00223-025-01407-4

Figure Lengend Snippet: The mRNA expression of ARNT2 on osteogenesis and adipogenesis of UCMSCs. A Osteogenesis of UCMSCs was indicated by ARS staining. (200 μm; scale bar) B , C Gene expression of RUNX2, OCN, OPN, BSP, and ARNT2 was analyzed by qRT-PCR assay. In contrast to the uninduced group, ∗∗ p < 0.01. D Adipogenesis of UCMSCs was detected using Oil Red O staining. (200 μm; scale bar) E , F Gene expression of FABP4 , PPARγ , and ARNT2 was tested by qRT-PCR assay. In contrast to the uninduced group, ∗∗ p < 0.01

Article Snippet: In the ARS staining procedure, cells were fixed with 4% paraformaldehyde (Phygene, China, Cat# PH0427) for 30 min following a 16-day osteogenic induction, followed by staining with ARS staining solution (Beyotime, China, Cat# C0148S) at ambient temperature for 30 min.

Techniques: Expressing, Staining, Gene Expression, Quantitative RT-PCR

Journal: Research

Article Title: Acousto-Electric Conversion Fiber Networks via Regional Activation of Schwann Cell-Derived Exosomes for Neurogenic Bone Regeneration

doi: 10.34133/research.0769

Figure Lengend Snippet: SC exosomes induced by acousto-electric conversion fiber networks promote BMSC bone differentiation in vitro: (A) Immunofluorescence staining of BMSCs. (B and C) Representative images and quantitative analysis of ALP staining. (D and E) Representative images and quantitative analysis of ARS staining. (F) RT-PCR analysis of osteogenic gene expression (NS, no significant difference; *** P < 0.05, n = 3).

Article Snippet: The extent of ECM mineralization was evaluated using ARS staining (Beyotime, Shanghai, China).

Techniques: In Vitro, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Gene Expression